Waters Hplc System Cleaning Procedure

To clearly lay down the procedure for cleaning.

Waters hplc system cleaning procedure.

Disconnect the column and attach a union and flow directly to waste. For information on other acquity uplc cleaning methods refer to the recommended cleaning mixtures for lc table in the con trolling contamination in ultraperformance lc ms and hplc ms systems document p n 715001307. Objective to lay down the procedure for operation of hplc waters alliance 2695 separation module scope to ensure that the instrument performs satisfactory and gives accurate and reproducible data. Controlling contamination in lc ms and hplc ms systems 715001307.

When preparing a system to run your method it is always important to prime up the system to ensure you get fresh solvents in all of the required lines. Maintaining your hplc uhplc uplc systems. Remove all solvent filters. To clean the system.

Responsibility quality control officer executive. Label bottles correctly with bottle content and filling date expiry date. Ultrapure water is water that has been purified through a system that targets contami nants detrimental to uplc ms and hplc ms systems. When working with any lc system setting it up and storing it is very important.

Place a b c and d seal wash and needle wash lines into a clean. 3 0 responsibility 3 1 doing. Ion exchange to remove any remaining ions. Water flush to clear customer s mobile phases from the system.

Residues or contaminations may block filters or capillaries. Use solvent inlet filters to protect the system from incoming particles. Cleaning decontamination columns general guidelines flow cells general. Cleaning mixtures for lc table in the controlling contamination in ultraperformance lc ms and hplc ms systems document p n 715001307.

Technical assistant 3 2 checking. No injections are needed with that. Reverse osmosis to remove most contaminants b. Use only hplc grade solvents and water filtered through 0 2.

The purification process should include all of the following steps. To clean the system. Water flush to clean acid from the system. 10 nh4oh in water this removes peg and other.

2 0 scope this procedure is applicable for of all high performance liquid chromatography installed in quality control laboratory. It is necessary to restore the system flow path to a near new level of cleanliness note. The water flushes are only needed to remove the previous solvent from the system. Executive manager 4 0 accountability head of the department 5 0 procedure 5 1 for system cleaning.